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Peripheral
Blood Lymphocytes
Description
Includes PHA-stimulated culture of peripheral blood lymphocytes
and preparation of Giemsa-banded chromosomes. Chromosome analysis
includes two banded karyotypes, detailed examination of ten cells,
and 20 cells total counted.
Indications
• congenital anomalies
• developmental delay
• short stature
• ambiguous genitalia
• recurrent miscarriages
Specimen Requirements
5-8 cc (2 cc for infants and small children) in green-top (sodium
heparin) tube; invert several times to mix blood. Culture of clotted
blood often does not result in spontaneously dividing cells necessary
for cytogenetic analysis. Other anticoagulants such as lithium heparin
may be harmful to the viability of the cells. Send to Great Lakes
Genetics at room temperature. May be refrigerated overnight. Specimen
cannot be frozen. Label tube with patient's name and laboratory
control number. Send to Great Lakes Genetics within 24 hours of
collection.
Reference Value
46,XX or 46,XY
Turn Around Time
2-7 days (generally 3 days)
CPT Codes
88262 - chromosome analysis, congenital disorders
88230 - tissue culture for chromosome analysis
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