|
Fluorescent
In-Situ Hybridization (FISH)
Description
FISH involves using whole chromosome paint probes, alpha-satellite
probes, or unique sequence probes. A FISH study includes color fluorescent
microscopy photographs and analysis of ten metaphases or 50 interphase
nuclei.
Indications
FISH studies are used to delineate complex chromosome rearrangements,
diagnose microdeletion syndromes, or demonstrate the presence of
molecular rearrangements characteristic of certain hematologic malignancies.
FISH studies are usually performed in conjunction with conventional
cytogenetic analysis, although occasionally a FISH study is performed
without G-banding chromosome analysis. Our cytogenetics laboratory
director is available at 414-475-5904 to discuss which FISH probe
would be appropriate for a particular clinical situation.
Probes Available
• chromosome paints
• alpha-satellites
• Wolf-Hirschhorn syndrome
• Williams syndrome
• Prader-Willi syndrome
• Angelman syndrome
• DiGeorge syndrome/velo-cardio-facial syndrome
• Miller-Dieker syndrome
• Smith-Magenis syndrome
• Kallmann syndrome/steroid sulfatase deficiency
• retinoblastoma
• major bcr/abl rearrangement t(9;22)
• minor bcr/abli rearrangement t(9;22)
• translocation t(15;17) for acute promyelocytic leukemia
• inversion (16) inv(p13q22) for acute myelomonocytic leukemia
FAB type M4e FAB type M3
Specimen Requirements
FISH studies may be performed on chromosome spreads or interphase
nuclei as prepared for conventional cytogenetic analysis. Specimen
requirements are as described for chromosome analysis on various
tissue types. Click the following links for specific specimen requirement
information:
Peripheral Blood Lymphocytes
Amniotic Fluid
Chorionic Villi
Skin Biopsy
Fetal/Placental Tissue
Bone Marrow
Lymph Node
Reference Value
Reference values depend upon the specific DNA probe used for a particular
clinical or cytogenetic indication.
Turn Around Time
5-10 days (generally 8 days)
CPT Codes
88271- molecular cytogenetic probe
88274 - Aneuvysion
88271x15; 88291; 88275- telomere probes
|
