|
Chorionic
Villi
Description
Includes direct preparation and short term culture of chorionic
villi as well as preparation of Giemsa-banded metaphase chromosomes.
Chromosome analysis includes two banded karyotypes, detailed analysis
of ten cells, and 20 cells total counted.
Indications
• advanced maternal age
• previous child with a chromosome abnormality
• familial chromosome rearrangement
• biochemical or molecular diagnosis of a familial genetic
disorder
Specimen Requirements
15-25 mg of chorionic villi. Use a stereomicroscope and sterile
forceps to remove any blood clots. Transfer the chorionic villi
to one or two screw-top sterile tubes and fill with sterile tissue
culture transport medium. Tissue culture medium is supplied by Great
Lakes Genetics. Please call 414-475-5904 to obtain medium. Label
tube(s) with patient's name and laboratory control number. Tissue
may be refrigerated. Specimen cannot be frozen. Send to Great Lakes
Genetics as soon as possible after tissue collection.
Reference Value
46,XX or 46,XY
Turn Around Time
1-2 days for direct analysis
1-2 weeks for short term culture
CPT Codes
88267 - chromosome analysis, chorionic villi
88235 - tissue culture for chorionic villi
|
